加味涤痰汤对卒中后认知障碍患者外周血miRNAs组合表达的影响研究Effects of modified Ditan Decoction on the combined expression of miRNAs in peripheral blood of patients with post-stroke cognitive impairment
罗玮;刘玲;戴丽星;曾华;
摘要(Abstract):
目的 观察卒中后认知障碍(PSCI)患者血清微小RNA(miRNAs)表达谱,验证目标miRNAs组合的表达情况及加味涤痰汤对其影响。方法 通过高通量测序方式测定PSCI患者及对照组(脑卒中后无认知障碍组和正常人组)血清miRNA表达情况,筛选与PSCI发病过程密切相关差异表达的miRNAs组合进行研究。收集30例PSCI患者给予加味涤痰汤口服,观察疗程8周,另收集同期认知功能正常的老年健康体检者30例作为对照组。采用荧光定量PCR法检测PSCI患者治疗前后及对照组血清目标 miRNAs的水平。结果 对筛选的20个差异miRNAs进行研究。与对照组比较,PSCI组治疗前血清miR-31、miR-93、miR-143、miR-146a、miR-107、miR-126表达显著升高(P<0.05),miR-29a,miRNA-34a表达显著降低(P<0.05)。与治疗前比较,PSCI组治疗后血清miR-29a、miRNA-34a表达升高(P<0.05),miR-31、miR-93、miR-143、miR-146a、miR-126表达显著降低(P<0.05),miR-107表达降低(P<0.05)。结论 通过检测血清miR-31、miR-93、miR-143、miR-146a、miR-107、miR-126及miR-29a、miRNA-34a表达水平可能有助于PSCI患者的早期诊断;加味涤痰汤能升高PSCI患者外周血血清miR-29a、miRNA-34a的水平,显著降低miR-31、miR-93、miR-143、miR-146a、miR-126的水平,使miR-107水平降低。
关键词(KeyWords): 卒中后认知障碍;miRNA组合;加味涤痰汤;早期诊断
基金项目(Foundation): 国家自然基金面上项目(81273648)
作者(Authors): 罗玮;刘玲;戴丽星;曾华;
DOI: 10.13463/j.cnki.jlzyy.2022.03.013
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