菌毒炎并治法对脓毒症大鼠免疫相关基因的影响
张照健;何英;李志军;
摘要(Abstract)：
目的:观察菌毒炎并治对脓毒症大鼠免疫相关基因的影响,探讨其免疫调节机制。方法:将大鼠分为模型组、菌毒炎组、对照组。用盲肠结扎穿孔术复制脓毒症模型。菌毒炎组腹腔注射抗生素、血必净注射液,凉膈散煎剂灌胃;对照组和模型组腹腔注射生理盐水。杀死大鼠后用芯片检测肝基因,筛选差异基因,查询功能并分类。结果:检测到差异表达的已知基因48个,其中免疫相关基因7个。结论:菌毒炎并治法对于脓毒症大鼠的改善可能与其对免疫相关基因的调控有关。
关键词(KeyWords)： 基因芯片;菌毒炎并治法;脓毒症;免疫相关基因;大鼠
基金项目(Foundation): 脓毒症大鼠基因改变及三证三法治疗机制实验研究天津市卫生局中医处资助项目(项目编号:07003)
作者(Authors): 张照健;何英;李志军;
DOI: 10.13463/j.cnki.jlzyy.2010.02.036
参考文献(References)：
- [1]Chaudry I H,Wichterman K A,Baue A E.Effect of sepsis on tissue adenine nucleo tide levels[J].Surgery,1979,85(2):205-211.
- [2]Drosten C,Gunter S,Preiser W,et al.Identification of A novel coronavirus in Patients with severe Acute Respiratorysyndrome [J].new Engl J med,2003,4:10.
- [3]Wang J,Cai Y,Xu H,et al.Expression of MR-P14 gene is frequently down-regulated in Chinese humanesophageal cancer[J]. CellResearch,2004,14(1):46-53.
- [4]Viemann D,Barczyk K,Vogl T,et al.MRP8/MRP14 impairs endothelial integrity and induces a caspase-dependent and-independent cell death program[J].Blood,2007,109(6):2453-2460.
- [5]Birkenmeier G.Targeting the protease inhibitor and immune modulatory function of human 2 macroglobulin[J].Mod Aspects Imunobiol, 2001,2:32-36.
- [6]Webb DJ,Gonias SL.A modified human alpha 2-macroglobulin derivative that binds tumor necrosis factor-alpha and interleukin -1 beta with high affinity in vitro and reverses lipopolysaccharide toxicity in vivo in mice[J].Lab Investig,1998,78(8):939-948.
- [7]Webb DJ,Wen J,Karns LR,et al.Localization of the binding site for transforming growth factor-βin human 2-macroglobulin to a 20- kDa peptide that also contains the bait region[J].Biol Chem, 1998,273:13339-13346.
- [8]Hertting O,Klalil A,Jaremko G,et al.Enhanced chemokine response in experimental acute Escherichia coli pyelonephritis in IL -1 beta-deficient mice[J].Clin Exp Immunol,2003,131(2): 225-233.
- [9]Akanksha Gupta,David T.Berg,Bruce G erlitz,et al.Role of Protein C in Renal Dysfunction after Polymicrobial Sepsis Gerlitz APC and Acute Renal Failure 2007[J].Soc Nephrol,2007,18:860-867.
- [10]刘霞,张巍,温浩.MSPGN中活化NF-κβp65mRNA、IL-1βmRNA、IL-4mRNA的检测及其意义[J].新疆医科大学学报,2005,28:730-733.
- [11]Nagy N,Legendre H,Engels O,et al.Refined prognostic evaluation in colon carcinoma a using immunohistochemical galectin fingerprinting[J].Cancer,2003,97(8):1849-1858.
- [12]Yang RY,Liu FT.Galectins in cell growth and apoptosis[J]. CellMol Life Sei,2003,60(2):267-276.
- [13]Liu FT,Patterson RJ.Wang·JL.Intracellular functions of galectins[J].Biochim BiophysActa.2002,1572(2-3):263-273.
- [14]Tribl B,Filipp D,B? deker H,et al.Pseudomonas pneumoniamediated sepsis induces expression of pancreatitis-associated protein-Ⅰin rat pancreas[J].Pancreas,2004,29(1):33-40.