涤痰通瘀方干预急性脑出血大鼠肝组织IRAK-M表达变化的影响IRAK-M in liver during acute cerebral hemorrhage and effects of Ditantongyu measure
王威;许晓英;马迪;赵海滨;唐杰;张秀静;王帅;翟瑶瑶;张淑静;
摘要(Abstract):
目的研究急性脑出血大鼠肝组织中IRAK-M表达的变化,并探讨涤痰通瘀方剂对急性脑出血大鼠肝损害的干预作用。方法通过建立SD大鼠急性脑出血模型,并随机分为中药组(酒大黄、胆南星、石菖蒲、蒲黄、三七、水蛭)、脑出血组、假手术组、正常组,每组分为24、48、72 h 3个时间亚组,检测每组肝脏组织IRAKM蛋白表达水平及肝组织肿瘤坏死因子-α(TNF-α)水平,并观察肝组织HE染色结果。结果脑出血模型组在造模后各个时间点IRAK-M蛋白表达水平较假手术组及正常组均明显降低(P<0.05);中药治疗组的IRAKM蛋白表达水平较脑出血模型组明显升高(P<0.05),与假手术组、正常组相比略有降低;中药治疗组及脑出血模型组肝脏IRAK-M蛋白表达水平与组织TNF-α水平呈负相关。结论 IRAK-M蛋白表达水平升高可能是抑制脑出血后诱发的LPS胞内信号转导炎性级联反应的重要因素;涤痰通瘀方剂可加强IRAK-M激酶活性,从而抑制炎症因子的释放,减轻急性脑出血大鼠肝脏损伤的程度。
关键词(KeyWords): 白细胞介素-1受体相关激酶-M;急性脑出血;肝损害;涤痰通瘀法;肿瘤坏死因子-α
基金项目(Foundation): 国家自然科学基金(81373590)
作者(Authors): 王威;许晓英;马迪;赵海滨;唐杰;张秀静;王帅;翟瑶瑶;张淑静;
DOI: 10.13463/j.cnki.jlzyy.2015.11.023
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