刘影哲;王春晓;潘祥宾;

• 1:黑龙江中医药大学附属第一医院
• 2:黑龙江中医药大学附属第二医院

摘要(Abstract)：

目的研究白虎加人参汤对糖尿病模型大鼠氧化应激TAOC水平及背根神经节(DRG)中TRPV1m RNA基因表达的影响。方法腹腔注射链脲佐菌素建立糖尿病大鼠模型。将造模成功大鼠随机分为模型对照组、白虎加人参汤组以及α-硫辛酸组。药物治疗8周后,采用酶联免疫法检测大鼠TAOC水平,RT-PCR法检测DRG中TRPV1 m RNA的表达。结果与模型对照组比较,白虎加人参汤组和α-硫辛酸组总抗氧化能力均升高(P<0.05),2组间比较,α-硫辛酸组TAOC水平更高,差异有统计学意义(P<0.05);白虎加人参汤组和α-硫辛酸组DRG中TRPV1 m RNA的表达与模型对照组比较均下调(P<0.01),组间比较差异无统计学意义(P>0.05)。结论白虎加人参汤能提高糖尿病大鼠血清TAOC水平,减轻氧化应激反应,具有一定的抗氧化能力,同时下调DRG中TRPV1 m RNA的表达,对糖尿病神经病变有一定的防治作用。

关键词(KeyWords)： 白虎加人参汤;糖尿病;氧化应激;TRPV1;大鼠

Abstract:

Keywords:

基金项目(Foundation): 黑龙江省教育厅基金项目(12521508);; 黑龙江省自然科学基金项目(H201314)

作者(Author): 刘影哲;王春晓;潘祥宾;

Email:

DOI:

参考文献(References)：

• [1]Brownlee M.Biochemistry and molecular cell biology of diabetic complications[J].Nature,2001(414):813-820.
• [2]Leibowitz G,Kaiser N,Cerasi E.β-Cell failure in type 2diabetes[J].J Diabetes Investig,2011,2(2):82-91.
• [3]Uma Singly Ishwarlal Jialal.Alpha-lipoic acid supplementation and diabetes[J].Nutr Rev,2008,66(11):646-657.
• [4]刘利群,毛定安,薄涛,等.川芎嗪对幼鼠惊厥性脑损伤脂质过氧化及抗氧化水平的影响[J].中国医院药学杂志,2008,28(4):253-256.
• [5]A.M.Barrett,M.A.Lucero,T.Le,R.L.Robinson,R.H.Dworkin,A.S.Chappell.Epidemiology,Public Health Burden,and Treatment of Diabetic Peripheral Neuropathic Pain:A Review[J].Pain Med,2007,8(2):50-62.
• [6]M.Otto,S.Bak,F.W.Bach,T.S.Jensen,S.H.Sindrup.Pain Phenomena and Possible Mechanisms in Patients with Painful Polyneuropathy[J].Pain,2003,101(1-2):187-192.
• [7]R.Eldor,I.Raz,A.Ben Yehuda,A.J.Boulton.New and Experimental Approaches to Treatment of Diabetic Foot Ulcers:A Comprehensive Review of Emerging Treatment Strategies[J].Diabet Med,2004,21(11):1161-1173.
• [8]L.Pradhan,C.Nabzdyk,N.D.Andersen,F.W.Lo Gerfo,A.Veves.Infl ammation and Neuropeptides:The Connection in Diabetic Wound Healing[J].Expert Rev Mol Med,2009(11):2.
• [9]S.K.Joshi,P.Honore,G.Hernandez,R.Schmidt,A.Gomtsyan,M.Scanio,M.Kort,M.F.Jarvis.Additive Antinociceptive Effects of the Selective Nav1.8 Blocker a-8 0 3 4 6 7 a n d S e l e c t i v e Tr p v 1 A n t a g o n i s t s i n R a t Infl ammatory and Neuropathic Pain Models[J].J Pain,2009,10(3):306-315.
• [10]O.Summ,P.R.Holland,S.Akerman,P.J.Goadsby.Trpv1Receptor Blockade Is Ineffective in Different in Vivo Models of Migraine[J].Cephalalgia,2011,31(2):172-180
• [11]H.L.Pan,Y.Q.Zhang,Z.Q.Zhao.Involvement of Lysophosphatidic Acid in Bone Cancer Pain by Potentiation of Trpv1 Via Pkcepsilon Pathway in Dorsal Root Ganglion Neurons[J].Mol Pain,2010(6):85.
• [12]Pabbidi RM,Yu SQ,Peng S,et al.Infl uence of TRPV1 on diabetes-induced alterations in thermal pain sensitivity[J].Mol Pain,2008(4):9.
• [13]M.Couto,Aide Diego,M.Perpina,et al.Cough Refl ex Testing With Inhaled Capsaicin and TRPVl Activation in Asthma and Comorbid Conditions[J].J Investig Allergol Clin Immunol,2013,3(5):289-301.
• [14]I.Devesa I,R.Planells-Cases,G.Femdndez-Ballester,et al.“Role of the transient receptor potential vanilloid I in inflammation and sepsis”[J].Journal of Inflammation Research,2011(4):67-81.
• [15]R.Y.Walder,R.Radhakrishnan,L.Loo,et al.TRPVl is important for mechanical and heat sensitivity in uninjured animals and development of heat hypersensitivity after muscle infl ammation[J].Pain,2012,153(8):1664-1672.
• [16]唐宗湘.温度敏感的TRP通道与中药药性四气理论[J].南京中医药大学学报,2015,31(7):301-304.
• [17]高琳,赵红霞,杨祯.热敏通道与中药的四气学说[J].中国中医基础医学,2014,20(12):1687-1690.
• [18]殷玉婷,董样.中医寒热体质与Thermo TRP通道相关性研究[J].江西中医药大学学报,2014,26(8):44-48.
• [19]杨幸怡,张晓晨,朱燕燕,等.姜黄素对TRP离子通道抑制作用的研究[J].热带医学杂志,2011,11(12):1359.
• [20]赵红霞,高琳,杨祯.热敏通道理论与引火归元[J].中国中医基础医学,2014,20(11):1507-1509.