王胜军;丁云录;李驰坤;李晓兵;初洪波;成光宇;

• 1:长春中医药大学附属医院

摘要(Abstract)：

目的探讨清肝降浊颗粒对非酒精性脂肪性肝病(NAFLD)模型大鼠肝脏的保护作用及其机制。方法将Wistar大鼠随机分为正常组、模型组、清肝降浊颗粒高、中和低剂量组(800、400和200 mg/kg),用高脂饲料喂养12周制备非酒精性脂肪性肝病模型,造模同时灌胃给药,每日1次。给药周期结束,检测血清TNF-α、IL-6、ADPN、Lep质量浓度及肝组织中FFA和TG摩尔浓度。结果造模成功后,模型组大鼠血清TNF-α、IL-6、Lep、肝组织中FFA和TG质量浓度明显升高;血清脂联素质量浓度明显降低,与正常对照组比较,均为P <0.01。在给予清肝降浊颗粒颗粒药物干预后,与模型组比较,清肝降浊颗粒高、中、低3个剂量组均可显著降低肝组织匀浆中TG摩尔浓度及升高血清中脂联素质量浓度(P <0.01或P <0.05);清肝降浊颗粒高、中2个剂量组均可显著降低肝组织匀浆中FFA摩尔浓度及血清中TNF-α、Lep质量浓度(P <0.01或P <0.05);清肝降浊颗粒高剂量组可显著降低血清中IL-6质量浓度(P <0.05)。结论清肝降浊颗粒改善非酒精性脂肪性肝病的作用机制,与降低血清TNF-α、IL-6、Lep质量浓度、肝组织FFA摩尔浓度,升高血清脂联素质量浓度相关。

关键词(KeyWords)： 清肝降浊颗粒;非酒精性脂肪性肝病;炎症因子;大鼠

Abstract:

Keywords:

基金项目(Foundation): 吉林省中医药科技项目(2017182)

作者(Author): 王胜军;丁云录;李驰坤;李晓兵;初洪波;成光宇;

Email:

DOI: 10.13463/j.cnki.jlzyy.2019.06.023

参考文献(References)：

• [1]潘良盈,赵曙光.非酒精性脂肪性肝病鼠科动物模型研究进展[J].肝脏,2015,20(5):412-414.
• [2]敬梦辉,蒋元烨,范伏岗,等.非酒精性脂肪性肝病动物实验模型最新研究进展[J].现代中西医结合杂志,2017,26(18):2049-2052.
• [3]范鸿儒,杨之娴,王雨晴,等.三草水煎剂对非酒精性脂肪肝大鼠治疗作用的实验研究[J].中华中医药学刊,2015,6(4):1672-1677.
• [4]隋晓丹,邓厚波,刘铁军.中医药防治非酒精性脂肪肝病的研究进展[J].世界华人消化杂志,2013,5(18):665-667.
• [5]黄海燕,辛永宁,姜曼,等.非酒精性脂肪性肝病动物实验模型研究进展[J].临床肝胆病杂志,2014,30(9):948-953.
• [6]TEMPLE J L,CORDERO P,LI J,et al.A guide to NonAlcoholic fatty liver disease in childhood and adolescence[J].Int J Mol Sci,2016,17(6):947
• [7]RAVI KANTH V V,SASIKALA M,SHARMA M,et al.Genetics of non-alcoholic fatty liver disease:From susceptibility and nutrient interactions to management[J].World J Hepatol,2016,8(20):827-837.
• [8]GOLABI P,LOCKLEAR C T,AUSTIN P,et al.Effectiveness of exercise in hepatic fat mobilization in non-alcoholic fatty liver disease:Systematic review[J].World J Gastroenterol,2016,22(27):6318-6327.
• [9]马晓燕,张岩.瘦素、脂联素对脂肪肝胰岛素抵抗影响机制的研究[J].中华中医药学刊,2013,31(12):2604-2605.
• [10]马赞颂,张莉,季光.瘦素与非酒精性脂肪肝的研究进展[J].中西医结合肝病杂志,2015,25(6):377-381.
• [11]ZELBER-SAGI S,RATZIU V,ZVIBEL I,et al.The association between adipocytokines and biomarkers for nonalcoholic fatty liver disease-induced liver injury:a study in the general population[J].Eur J Gastroenterol Hepatol,2012,24(3):262-269.
• [12]王翠华,岳妍.瘦素、脂联素与非酒精性脂肪性肝病关系的研究进展[J].临床肝胆病杂志,2015,31(8):1351-1354.
• [13]STACHOWSKA E,RYTERSKA K,MACIEJEWSKA D,et al.Nutritional strategies for the individualized treatment of Non-Alcoholic fatty liver disease(NAFLD)based on the Nutrient-Induced insulin output ratio(NIOR)[J].Int J Mol Sci,2016,17(6):E1192.
• [14]NI Y,ZHUGE F,NAGASHIMADA M,et al.Nobel action of carotenoids on Non-Alcoholic fatty liver disease:macrophage polarization and liver homeostasis[J].Nutrients,2016,8(7):E391.
• [15]SUZUKI-KEMURIYAMA N,MATSUZAKA T,KUBAM,et al.Different effects of eicosapentaenoic and docosahexaenoic acids on atherogenic High-Fat DietInduced Non-Alcoholic fatty liver disease in mice[J].PLoSOne,2016,11(6):E0157580.