中药复方糖肾安对实验性2型糖尿病大鼠肾脏ROS水平及Nrf2、HO-1表达的影响Tang Shenan decoction on ROS levels and Nrf2,HO-1 expression in experimental type 2 diabetic rats kidney
孙伟娜;王镁;
摘要(Abstract):
目的探讨中药复方糖肾安对实验性2型糖尿病大鼠肾脏ROS水平、Nrf2、HO-1表达的影响。方法70只健康SD大鼠随机分为正常组(n=10)和造模组(n=60)。造模组大鼠高糖高脂饮食4周后一次性腹腔注射STZ(35 mg/kg)建立2型糖尿病大鼠模型,将成模的大鼠随机分为模型组、糖肾安低、中、高剂量组、西药组。药物治疗8周后,用流式细胞仪检测ROS水平,用定量RT-PCR、Western blot检测Nrf2、HO-1表达情况。结果各治疗组ROS水平均高于正常组(P<0.01),糖肾安组ROS水平均明显低于模型组(P<0.01)。各治疗组Nrf2、HO-1表达均低于正常组(P<0.01),糖肾安中、高剂量组的表达较高,高于模型组(P<0.01)。结论中药复方糖肾安可能通过激活抗氧化应激Nrf2/ARE通路,诱导糖尿病大鼠肾脏Nrf2、HO-1的表达,降低ROS水平,改善肾脏炎症反应,从而起到肾脏保护作用。
关键词(KeyWords): 糖肾安煎剂;厄贝沙坦;氧化应激;糖尿病大鼠
基金项目(Foundation): 沈阳市科技局项目(F14-231-1-11)
作者(Author): 孙伟娜;王镁;
Email:
DOI: 10.13463/j.cnki.jlzyy.2016.12.019
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