郝利珍;高兴州;常宏;姜厚望;王磊;朱佳杰;王谦;王晓波;柴欣楼;

• 1:北京中医药大学基础医学院

摘要(Abstract)：

目的研究槲皮素对过氧化氢(H2O2)诱导脐静脉内皮细胞(HUVECs)凋亡的影响。方法体外培养脐静脉内皮细胞,H2O2作用于HUVECs诱导其凋亡,采用MTT法检测细胞活力,流式细胞术检测细胞凋亡率,Western-blot检测GRP78、XBP1及caspase-3蛋白表达。结果 H2O2呈剂量依赖性降低内皮细胞活力,并引起细胞凋亡。其中500μmol/L H2O2作用于细胞后,与正常组相比,细胞活力明显受到抑制,且GRP78、XBP1、caspase-3等蛋白含量明显增加(P<0.01);槲皮素预处理后,随着槲皮素浓度增加,细胞活力随之增加;与模型组相比,10μmol/L槲皮素能够显著抑制细胞凋亡,GRP78、XBP1、caspase-3等表达降低(P<0.01)。结论槲皮素能抑制H2O2诱导的内皮细胞凋亡,该作用可能与下调GRP78、XBP1、caspase-3等表达有关。

关键词(KeyWords)： 槲皮素;细胞凋亡;GRP78;XBP1;caspase-3

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81002542)

作者(Author): 郝利珍;高兴州;常宏;姜厚望;王磊;朱佳杰;王谦;王晓波;柴欣楼;

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