重组人参脂质转运蛋白表达载体构建及抗疫病活性研究Recombination ginseng lipid transfer protein gene expression vector to construct and analysis of its antifungal activity
才可新;秦晓晔;李帅军;王敏;张鹏飞;赵雨;曲毅;
摘要(Abstract):
目的研究人参脂质转运蛋白(Lipid transfer protein,LTP)对人参疫病菌的抑制作用。方法采用RT-PCR技术克隆人参LTP基因,构建表达载体,并转化到大肠杆菌中,IPTG诱导表达。采用Ni–NTA亲和层析纯化目的蛋白,纸片扩散法验证目的蛋白的抗菌活性。结果人参LTP基因全长为363个碱基,编码120个氨基酸;目的蛋白经纯化后,SDS-PAGE电泳检测为单一条带;抑菌试验表明,重组LTP可明显抑制人参疫病菌丝的生长。结论人参LTP对人参疫病菌具有显著抑制作用。
关键词(KeyWords): 人参;脂质转运蛋白;抑菌活性
基金项目(Foundation): 吉林省省级经济结构战略调整引导资金专项项目(2014N155);; 长春市科技计划项目(2013077)
作者(Author): 才可新;秦晓晔;李帅军;王敏;张鹏飞;赵雨;曲毅;
Email:
DOI: 10.13463/j.cnki.jlzyy.2016.12.021
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