才可新;秦晓晔;李帅军;王敏;张鹏飞;赵雨;曲毅;

• 1:长春中医药大学中医药与生物工程研究开发中心
• 2:吉林省食品药品安全监测中心

摘要(Abstract)：

目的研究人参脂质转运蛋白(Lipid transfer protein,LTP)对人参疫病菌的抑制作用。方法采用RT-PCR技术克隆人参LTP基因,构建表达载体,并转化到大肠杆菌中,IPTG诱导表达。采用Ni–NTA亲和层析纯化目的蛋白,纸片扩散法验证目的蛋白的抗菌活性。结果人参LTP基因全长为363个碱基,编码120个氨基酸;目的蛋白经纯化后,SDS-PAGE电泳检测为单一条带;抑菌试验表明,重组LTP可明显抑制人参疫病菌丝的生长。结论人参LTP对人参疫病菌具有显著抑制作用。

关键词(KeyWords)： 人参;脂质转运蛋白;抑菌活性

Abstract:

Keywords:

基金项目(Foundation): 吉林省省级经济结构战略调整引导资金专项项目(2014N155);; 长春市科技计划项目(2013077)

作者(Author): 才可新;秦晓晔;李帅军;王敏;张鹏飞;赵雨;曲毅;

Email:

DOI: 10.13463/j.cnki.jlzyy.2016.12.021

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