蔡娲;马文;王观涛;沈卫东;

• 1:上海中医药大学附属曙光医院针灸科
• 2:上海中医药大学附属曙光医院针刺麻醉研究所

摘要(Abstract)：

目的通过探究针刺对脑卒中后抑郁(PSD)大鼠血清丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)表达的影响,探讨针刺治疗PSD的可能机制。方法将SD大鼠随机分为正常组、模型组、针刺组,8只/组。采用中脑动脉闭塞(MCAO)和慢性不可预知应激法(CUMS)建立PSD模型。针刺组针刺百会、神庭,1次/d,共治疗28 d。观察大鼠糖水偏爱比及旷场实验行为;Elisa法检测大鼠血清SOD、MDA、GSH含量。结果与正常组比较,模型组大鼠的糖水偏爱比(P <0.01),移动次数显著减少(P <0.001),MDA表达水平显著上升(P <0.05),SOD表达水平显著降低(P <0.05);与模型组相比,针刺组大鼠的糖水偏爱比(P <0.01)、移动次数显著上升(P <0.001),MDA表达水平显著减少(P <0.05),SOD、GSH表达水平显著升高(P <0.05)。结论针刺能够通过增加抗氧化物SOD、GSH水平,同时减少氧化产物MDA的产生来减少氧化应激损伤,从而减轻PSD的抑郁症状。

关键词(KeyWords)： 针刺;脑卒中后抑郁;氧化应激;丙二醛;超氧化物歧化酶;谷胱甘肽

Abstract:

Keywords:

基金项目(Foundation): 上海市科学技术委员会科研计划项目(16401970402/18401970601);; 上海市卫生和计划生育委员会中医药科技创新项目(ZYKC201701001);; 上海中医药大学研究生创新培养项目(Y201805)

作者(Author): 蔡娲;马文;王观涛;沈卫东;

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DOI:

参考文献(References)：

• [1]游毅,阎振国,陈卉,等.卒中后抑郁的中医药研究进展[J].吉林中医药,2018,38(8):980-983.
• [2]HACKETT M L,YAPA C,PARAG V,et al.Frequency of depression after stroke:a systematic review of observational studies[J].Stroke,2005,36(6):1330-1340.
• [3]李雅书.中药配合针刺治疗脑卒中后抑郁症36例[J].长春中医药大学学报,2008,1(3):301.
• [4]刘丹,朱永志,张迪.针刺从痰论治对卒中后抑郁(PSD)大鼠5-羟色胺1A受体(5-HT1_AR)及脑源性神经营养因子(BDNF)变化的影响[J].黑龙江中医药,2011,40(1):27-28.
• [5]王强.健脑安神针刺法对脑卒中后抑郁大鼠5-羟色胺受体1A(5-HT1A)、5-羟色胺受体2A(5-HT2A)mRNA水平表达的影响[D].长春:长春中医药大学,2009:50.
• [6]王冠超.通督治郁针法对PSD患者血清5-HT、NE和BDNF水平影响的研究[D].合肥:安徽中医药大学,2014:62.
• [7]章显宝,汪燕,王震,等.针刺对卒中后抑郁大鼠5-HTT、5-HT1AR、NEα2R蛋白表达的影响[J].北京中医药大学学报,2016,39(9):783-788.
• [8]朱婷婷.通督治郁针法对卒中后抑郁患者血清GABA水平的影响[D].合肥:安徽中医药大学,2014:57.
• [9]郭爱松,金宏柱.针刺治疗卒中后抑郁症近况[J].针灸临床杂志,2007,1(11):52-54.
• [10]邓懿函.针刺治疗卒中后抑郁的临床疗效及对血浆中同型半胱氨酸含量的影响[D].福州:福建中医药大学,2015:36.
• [11]WALLACE D C.A mitochondrial paradigm of metabolic and degenerative diseases,aging,and cancer:a dawn for evolutionary medicine[J].Annual review of genetics,2005,39(1):359-407.
• [12]岑娟,张峰,姬汴生.缺血性中风抗氧化治疗的临床问题及研究进展[J].中国临床药理学与治疗学,2013,18(4):448-454.
• [13]DE MORAIS H,DE SOUZA C P,DA SILVA L M,et al.Increased oxidative stress in prefrontal cortex and hippocampus is related to depressive-like behavior in streptozotocin-diabetic rats[J].Behavioural brain research,2014,258(1):52-64
• [14]RAWDIN B J,MELLON S H,DHABHAR F S,et al.Dysregulated relationship of inflammation and oxidative stress in major depression[J].Brain,behavior,and immunity,2013,31(2):143-152.
• [15]ROUGEMONT M,DO K Q,CASTAGNE V.New model of glutathione deficit during development:Effect on lipid peroxidation in the rat brain[J].Journal of neuroscience research,2002,70(6):774-783.
• [16]SCHAFER F Q,BUETTNER G R.Redox environment of the cell as viewed through the redox state of the glutathione disulfide/glutathione couple[J].Free radical biology&medicine,2001,30(11):1191-1212.
• [17]SHI ZHENG ZHENG,OSEI-FRIMPONG J,KALA G,et al.Glutathione synthesis is essential for mouse development but not for cell growth in culture[J].Proceedings of the National Academy of Sciences of the United States of America,2000,97(10):5101-5106.
• [18]LONGA E Z,WEINSTEIN P R,CARLSON S,et al.Reversible middle cerebral artery occlusion without craniectomy in rats[J].Stroke,1989,20(1):84-91.
• [19]ZHU SHENG HUA,SHI RUO YANG,WANG JUN HUI,et al.Unpredictable chronic mild stress not chronic restraint stress induces depressive behaviours in mice[J].Neuroreport,2014,25(14):1151-1155.
• [20]PACE T W,HU FANG,MILLER A H.Cytokine-effects on glucocorticoid receptor function:relevance to glucocorticoid resistance and the pathophysiology and treatment of major depression[J].Brain,behavior,and immunity,2007,21(1):9-19.
• [21]HARVEY P W.Adrenocortical endocrine disruption[J].The Journal of steroid biochemistry and molecular biology,2016,155(11):199-206.
• [22]RADDATZ D,TOTH S,SCHWORER H,et al.Glucocorticoid receptor signaling in the intestinal epithelial cell lines IEC-6 and Caco-2:evidence of inhibition by interleukin-1beta[J].International journal of colorectal disease,2001,16(6):377-383.
• [23]KOO J W,RUSSO S J,FERGUSON D,et al.Nuclear factorkappa B is a critical mediator of stress-impaired neurogenesis and depressive behavior[J].Proceedings of the National Academy of Sciences of the United States of America,2010,107(6):2669-2674.
• [24]LIU RONG PEI,ZOU MING,WANG JIAN YONG,et al.Paroxetine ameliorates lipopolysaccharide-induced microglia activation via differential regulation of MAPK signaling[J].Journal of neuroinflammation,2014,11(4):2-7.
• [25]LEWIS-TUFFIN L J,CIDLOWSKI J A.The physiology of human glucocorticoid receptor beta(hGRbeta)and glucocorticoid resistance[J].Annals of the New York Academy of Sciences,2006,10(06):1-9.
• [26]WEBSTER J C,OAKLEY R H,JEWELL C M,CIDLOWSKIJ A.Proinflammatory cytokines regulate human glucocorticoid receptor gene expression and lead to the accumulation of the dominant negative beta isoform:a mechanism for the generation of glucocorticoid resistance[J].Proceedings of the National Academy of Sciences of the United States of America,2001,98(12):6865-6870.