邢玮;王政霖;王宏亮;吕甜甜;吴晏;王伟;韩静;

• 1:北京中医药大学

摘要(Abstract)：

目的探寻活血解毒方关于糖尿病大鼠视网膜组织Notch信号通路的影响。方法将大鼠随机分为模型组、正常组、活血解毒方组。采用一次性腹腔注射链脲佐霉素65 mg/kg,建立糖尿病大鼠模型。灌胃12周后,取大鼠视网膜组织,应用免疫组化技术测定Notch1、Dll4的表达变化;提取大鼠视网膜组织中的总蛋白,应用Western-blot技术检测Hes1的表达变化。结果 1)免疫组化结果显示,模型组Notch1、Dll4表达量高于正常组(P<0.05),活血解毒方组Notch1、Dll4表达量低于模型组(P<0.05);2)Western-blot的结果显示,模型组Hes1表达高于正常组(P<0.05),活血解毒方组Hes1表达低于模型组。结论活血解毒方可能通过抑制糖尿病大鼠视网膜组织中的Notch信号通路,减少Notch1、Dll4、Hes1的表达,阻止糖尿病视网膜病变的恶化。

关键词(KeyWords)： 糖尿病视网膜病变;活血解毒方;Notch信号通路

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金课题(81673705)

作者(Authors): 邢玮;王政霖;王宏亮;吕甜甜;吴晏;王伟;韩静;

DOI: 10.13463/j.cnki.jlzyy.2018.07.021

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