张晓晶;钟相根;贾旭;邓秀兰;王青青;郑子安;王坦;

• 1:北京中医药大学基础医学院

摘要(Abstract)：

目的:探讨左归丸对实验性自身免疫性脑脊髓炎小鼠(experimental autoimmune encephalomyelitis,EAE)IL-23/IL-17炎性轴的影响。方法:建立髓鞘少突胶质细胞糖蛋白_(35-55)(myelin oligodendrocyte glycoprotein 35-55,MOG_(35-55))诱导C57BL/6小鼠EAE模型,随机分为模型组、泼尼松组、左归丸组,并设正常对照组。观察小鼠的发病情况、神经功能评分及体质量变化,HE染色观察CNS病理损伤程度,实时荧光定量PCR法检测小鼠脑组织IL-23 p19、IL-17 mRNA表达。结果:与正常对照组比较,模型组脑组织IL-23 p19、IL-17mRNA表达显著升高。左归丸及泼尼松组小鼠脑组织IL-23 p19、IL-17 mRNA表达比模型组低(P<0.05,P<0.01)。结论:左归丸能够抑制IL-23/IL-17炎性轴而起到抗炎作用。

关键词(KeyWords)： 多发性硬化;实验性自身免疫性脑脊髓炎;左归丸;IL-23/IL-17炎性轴;小鼠

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30901909);; 高等学校博士学科点专项科研基金新教师基金项目(200800261037)

作者(Author): 张晓晶;钟相根;贾旭;邓秀兰;王青青;郑子安;王坦;

Email:

DOI: 10.13463/j.cnki.jlzyy.2013.06.044

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