郑丽红;王馨;王琳晶;王楠楠;王海强;

• 1:黑龙江中医药大学附属第二医院
• 2:黑龙江中医药大学附属第一医院

摘要(Abstract)：

目的探讨愈肠栓对实验性溃疡性结肠炎大鼠炎症因子的影响。方法将实验大鼠随机分为正常对照组、模型组、柳氮磺吡啶栓组、愈肠栓组。治疗2周后,观察大鼠疾病活动指数(DAI)、结肠组织病理改变及血清TNF-α、IL-1β、IL-4、IL-10含量变化。结果与正常对照组比较,模型组大鼠DAI评分、血清中TNF-α、IL-1β水平明显升高,IL-4、IL-10水平明显降低,差异有统计学意义(P <0.01);与模型组比较,柳氮磺吡啶栓组、愈肠栓组DAI评分、TNF-α、IL-1β明显降低,IL-4、及IL-10水平明显升高,差异有统计学意义(P <0.05或P <0.01);柳氮磺吡啶栓组与愈肠栓组比较差异无统计学意义。结论愈肠栓能有效改善TNBS诱导的溃疡性结肠炎模型大鼠症状,其机制可能是通过降低血清炎症因子TNF-α、IL-1β含量,上调抑炎因子IL-4、IL-10的水平,从而达到缓解大鼠溃疡性结肠炎的作用。

关键词(KeyWords)： 愈肠栓;溃疡性结肠炎;炎症因子

Abstract:

Keywords:

基金项目(Foundation): 黑龙江省自然科学基金项目(H2016077);黑龙江省自然科学基金(H2017061);; 黑龙江中医药大学校基金项目(201301);; 黑龙江省卫生计生委科研基金(2018235)

作者(Author): 郑丽红;王馨;王琳晶;王楠楠;王海强;

Email:

DOI: 10.13463/j.cnki.jlzyy.2019.06.022

参考文献(References)：

• [1]UNGARO R,MEHANDRU S,ALLEN P B,et al.Ulcerative colitis[J].Lancet,2017,389(10080):1756-1770.
• [2]UDAI P SINGH,NARENDRA P SINGH,E ANGELAMURPHY,et al.Chemokine and cytokine levels in inflammatory bowel disease patients[J].Cytokine,2016(77):44-49.
• [3]MURANO M,MAEMURA K,HIRATA I,et al.Therapeutic effect of intracolonically administered nuclear factor kappa B(p65)antisense oligonucleotide on mouse dextran sulphate sodium(DSS)-induced colitis[J].Clin Exp Immunol,2000(120):51-58.
• [4]POLI?SKA B,MATOWICKA-KARNA J,KEMONA H.The cytokines in inflammatory bowel disease[J].Postepy Hig Med Dosw,2009(63):389-394.
• [5]SANDS B E,KAPLAN G G.The role of TNFalpha in ulcerative colitis[J].J Clin Pharmacol,2007,47(8):930-941.
• [6]OLSEN T,GOLL R,CUI G,et al.Tissue levels of tumor necrosis factor-alpha correlates with grade of inflammation in untreated ulcerative colitis[J].Scand.J.Gastroenterol,2007,42(11):1312-1320.
• [7]WU P,GUO Y,JIA F,et al.The Effects of Armillarisin A on Serum IL-1βand IL-4 and in Treating Ulcerative Colitis[J].Cell Biochem.Biophys,2015,72(1):103-106.
• [8]GRIGA T,HEBLER U,VOIGT E,et al.Interleukin-4 inhibits the increased production of vascular endothelial growth factor by peripheral blood mononuclear cells in patients with inflammatory bowel disease[J].Hepatogastroenterology,2000(36):1604-1607.
• [9]TU L,CHEN J,ZHANG H,et al.Interleukin-4 Inhibits Regulatory T Cell Differentiation through Regulating CD103+Dendritic Cells[J].Front Immunol,2017(8):214.
• [10]FONSECA-CAMARILLO G,FURUZAWA-CARBALLEDAJ,MARTíNEZ-BENíTEZ B,et al.Intelukin-10 expression with immunorreglatory function in the mucosa of patients with ulcerative colitis[J].Rev Gastroenterol Mex,2011,76(2):113-119.
• [11]朱春洋,周强,赵鲁卿.张声生教授从内痈论治溃疡性结肠炎经验[J].天津中医药,2016,33(11):641-643.
• [12]何润.姜树民治疗溃疡性结肠炎经验介绍[J].新中医,2017,49(11):177-178.
• [13]毛堂友,李军祥,史瑞.李军祥教授治疗溃疡性结肠炎临证经验[J].辽宁中医药大学学报,2018,20(5):104-106.